| Qualitatively, no difference was observed in the cytotoxicity of unheated, heated and ultra filtered culture supernatants of isolates. |
| The culture supernatants, though produced cytopathic effect in various tissue cultures, failed to have any deleterious effect on the worms. |
| Cytokine concentrations were measured in culture supernatants by enzyme-linked immunoadsorbent assay after centrifugation. |
| The culture supernatants were analysed and butyric acid identified as the potentially toxic material. |
| Agglutination reaction was carried out using the reagents with the stool supernatants. |
| After 5 days, supernatants were collected and used to infect fresh cell cultures. |
